Final Applied Lab Project The Effect of low pH on Enzyme Activity

Gentlepeople,Below (also attached in a word document) is a project for my Biology class. I am submitting this because I am mentally overloaded, and am willing to share the burden with anybody who would like to take a whack at this. Please review the information below and let me know what you think!IMPORTANT: I know that this project has been completed on CourseHero in the past, but I am looking for a completely fresh, NON-PLAGIARIZED, new version of this project. Please contact me with any questions and discussion of price!Instructions Final Applied Lab Project (1 credit Lab Component)Addresses course outcomes 1-4:recognize and explain how the scientific method is used to solve problemsmake observations and discriminate between scientific and pseudoscientific explanationsweigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific methoduse knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusionsThis is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply the principles of the scientific method.The Effect of low pH on Enzyme ActivityDesign an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources.Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.Lab MaterialsYou may need all or some of the following, depending on your experimental design:Materials from your lab kit:pH paperhydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)plastic beakers or cupsvinegaryeast (can be purchased at grocery store if more is needed)balloonsplastic bottlemarker for labeling of beakersYou may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate if you chose an enzyme other than catalase).Outline (10 points) submit in assignment folder in week 7.Include the following in your outline:Name of enzyme you will useName of organism (if applicable)The substrateMethod for measuring enzyme activityTreatment: acidic fluid(s), pH, length of exposure, how you will treat your samplesThe control(s) in the experimentSample sizeHypothesisHow you will present your data (table and/or type of graph)Anything else you would like to get feedback on before you start your experimentWrite a paper that includes the following:1. Title page: descriptive title, your name, course name, semester2. Introduction: general background information about enzymes and specific information about your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (15 points).3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points).4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers (20 points)5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (15 points)6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment? (10 points)7. Include a list of references to all information sources used in APA format (5 points).Avoid PlagiarismIt is very important to write with your own words. If you do copy one or two sentences directly (use sparingly), use quotation marks (“) around the copied text, followed by an in-text reference. All information sources need to be included in the reference list and as in-text references. Plagiarism will be reported to the proper UMUC authority. Guidance on how to avoid plagiarism can be found here: UMUC’s How to Avoid PlagiarismSubmissionSubmit your final applied lab project in the assignment folder by the due date specified in the course schedule.Hide RubricsRubric Name: Final Applied Laboratory ProjectCriteria Excellent (90-100%)100 points Good (75-89%)89 points Adequate (50-74%)74 points Unacceptable (0-49%)49 pointsOutline 10 points (Due in week 7) Outline includes all the required components and planned experiment meets requirements for the assignment and is clearly and accurately described. Submitted on time.9-10 pointsOutline missing one or two of the required components, and/or planned experiment does not meet one of requirements for the assignment and/or minor issues with clarity and accuracy.7-8 pointsOutline missing several of the required components, and/or planned experiment does not meet several of the requirements for the assignment and/or major issues with clarity and accuracy.5-6 pointsOutline missing most of the required components and planned experiment does not meet the requirements of the assignment.0-4 pointsIntroduction, Question and Hypothesis (15 points) Background information about enzymes in general and about specific enzyme used in project is clearly and accurately written. Questions and hypothesis are specific, relevant and clearly stated.14-15 pointsBackground information about enzymes in general and about specific enzyme is somewhat unclear and/or inaccurate. Questions and hypothesis could be more specific, relevant and clearly stated.11-13 pointsMissing background information about enzymes in general or about specific enzyme used in project or questions and hypothesis.7-12 pointMissing background information about enzymes in general and about specific enzyme used in project and questions/hypothesis.0-6 pointsExperimental Design, Materials and Methods (20 points) Experiment is designed to directly test the hypothesis; description of experiment is detailed and well written and includes all materials and methods used.18-20 pointsExperiment is mostly designed to test the hypothesis and/or description of experiment is somewhat inaccurate and/or some information about materials and methods used is missing and/or minor problems with clarity an organization.15-17 pointsExperiment is barely designed to test the hypothesis and/or description of experiment is inaccurate and/or most of the materials and methods used are missing and/or major problems with clarity an organization.10-14 pointsExperiment is not designed to test hypothesis and/or description of experiment and materials and methods used are missing.0-9 pointsResults (20 points) Results are clearly and accurately presented in a table and/or graph format.18-20 pointsResults are presented, but minor problems with clarity and/or accuracy.15-17 pointsResults are described, but major problems with clarity and/or accuracy and/or results not presented in table or graph.10-14 pointsResults are not included0-9 pointsDiscussion of Results (15 points) A clear, accurate and well organized discussion of results that demonstrates good knowledge of enzymes.14-15 pointsMinor problems with clarity, accuracy or organization and/or demonstrates some gaps in knowledge of enzymes.15-17 pointsMajor problems with clarity, accuracy or organization or demonstrates minimal knowledge of enzymes.10-14 pointsMissing discussion of results, or major problems with clarity, accuracy or organization and missing understanding of enzymes.0-9 pointsConclusion ( 10 points ) A well stated conclusion that is accurate and specific and directly related to the data collected. Clearly stated whether or not the results support the hypothesis. Relevant suggestion for improvement of experiment.9-10 pointsConclusion stated, but slightly inaccurate, and/or not directly related to data collected and/or suggestion for improvement of experiment not very relevant.7-8 pointsConclusion stated, but inaccurate and/or barely related to data collected and/or missing suggestion for improvement of experiment.5-6 pointsMissing conclusion, only suggestion for improvement of experiment.0-4 pointsCitations (5 points) All references used are included and in correct APA format, in-text references are included.5 pointsAll references are included, but a few minor mistakes in APA format, and/or missing in-text references.4 pointsMissing one or several references and/or major mistakes in APA format.3 pointNo references included or references not in APA format.0-2 pointsGrammar and Spelling (5 points) Less than 3 minor spelling and grammatical errors5 pointsBetween 4 and 7 minor spelling and grammatical errors4 pointsBetween 8 and 12 spelling and grammatical errors3 pointMore than 13 spelling and grammatical errors0-2 pointsOverall Score Level 490 or more Level 375 or more Level 250 or more Level 10 or more******************************************************************

Instructions

Final Applied Lab
Project (1 credit Lab Component)
Addresses
course outcomes 1-4:

recognize and explain how the scientific method is
used to solve problems
make observations and discriminate between scientific
and pseudoscientific explanations
weigh evidence and make decisions based on strengths
and limitations of scientific knowledge and the scientific method
use knowledge of biological principles, the
scientific method, and appropriate technologies to ask relevant
questions, develop hypotheses, design and conduct experiments, interpret
results, and draw conclusions

This is the
culminating lab assessment in BIOL 103.It is designed to
assess your ability to apply the principles of the scientific method.
The Effect of low pH
on Enzyme Activity
Design an experiment
in which you will test the effect of an acidic fluid on enzymatic activity.
Recall: enzymes are proteins! To complete this project, it may be useful for
you to first review the Scientific Method Tutorial, the OLI module about pH
and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As
you review Lab 4, you will be reminded that there are several factors that
impact enzymatic activity: pH, temperature, and amount of reagent. It is
OK to use the same enzyme/substrate/method as you did in lab 4 (but modify
the treatment), or you can search on-line to find a different
enzyme/substrate/method for measuring enzyme activity for your project
(include all references).
As you design your
experiment for this project, please remember that you are trying to examine
how an acidic fluid will modify the outcome of an enzymatic
reaction. To successfully complete this project, you will need to
identify the question(s) being asked in your experiment and the hypothesis
that you are testing. In your experimental design, you must clearly
explain what you are doing. That means that you will need to identify
the enzyme, the substrate, the acidic fluid used as treatment, the control
treatment and the method of measuring enzyme activity, as well as explain
your experimental protocol. You must also thoroughly explain how the
acidic fluid impacted enzyme activity based on the results from your own
experiment as well as knowledge of enzymes and pH from the OLI modules, lab
manual and potentially additional information sources.
Hint: Keep in mind that the acid will change
the environmental conditions of the experiment (for example, a low pH value
could change the shape of the active site on the enzyme protein), without
directly participating in the reaction.
Lab Materials
You may need
all or some of the following, depending on your experimental design:
Materials from your
lab kit:

pH paper
hydrogen peroxide solution (you can purchase this at
a pharmacy if you have used up the bottle that came with the lab kit)
plastic beakers or cups
vinegar
yeast (can be purchased at grocery store if more is
needed)
balloons
plastic bottle
marker for labeling of beakers

You may choose to
use additional materials (different acidic fluids and/or different organisms
and/or different substrate if you chose an enzyme other than catalase).
Outline (10 points) submit in assignment
folder in week 7.
Include the
following in your outline:

Name of enzyme you will use
Name of organism (if applicable)
The substrate
Method for measuring enzyme activity
Treatment: acidic fluid(s), pH, length of exposure,
how you will treat your samples
The control(s) in the experiment
Sample size
Hypothesis
How you will present your data (table and/or type of
graph)
Anything else you would like to get feedback on
before you start your experiment

Write a paper that
includes the following:
1. Title page:
descriptive title, your name, course name, semester
2. Introduction:
general background information about enzymes and specific information about
your chosen enzyme, the question(s) that you are asking and a clear
hypothesis for your experiment (15 points).
3. Design an
experiment. Provide a detailed description of the materials and methods used
to conduct the experiment. Identify control and experimental samples, as well
as independent and dependent variables. Also include the methods used for
data collection and analysis (20 points).
4. Conduct the
experiment and record your results. What did you observe? Present your
data in table and/or graph format. Remember to label everything and include
the unit of measure with all numbers (20 points)
5. Use your
knowledge of enzymes and pH to interpret and discuss your results. It may be
necessary for you to refer to the OLI course modules, lab manual and/or use
additional information resources. What effect does the acidic
treatment have on enzyme activity? Did you get the expected results?
Explain. (15 points)
6. State a
specific and accurate conclusion. Is your hypothesis supported by the
results? Looking back, how could you have improved your experiment? (10
points)
7. Include a list of
references to all information sources used in APA format (5 points).
Avoid Plagiarism
It is very important
to write with your own words. If you do copy one or two sentences
directly (use sparingly), use quotation marks (“) around the copied
text, followed by an in-text reference. All information sources need to be
included in the reference list and as in-text references. Plagiarism will be
reported to the proper UMUC authority. Guidance on how to avoid plagiarism
can be found here:.umuc.edu/prog/ugp/ewp_writingcenter/modules/plagiarism/start.html”>UMUC’s How to Avoid Plagiarism
Submission
Submit your final
applied lab project in the assignment folder by the due date specified in the
course schedule.

Hide Rubrics
Rubric Name: Final
Applied Laboratory Project

Criteria

Excellent (90-100%)
100 points

Good (75-89%)
89 points

Adequate (50-74%)
74 points

Unacceptable (0-49%)
49 points

Outline
10 points (Due in week 7)

Outline includes all the
required components and planned experiment meets requirements for the
assignment and is clearly and accurately described. Submitted on time.
9-10 points

Outline missing one or two of
the required components, and/orplanned experiment does not meet
one of requirements for the assignment and/orminor issues with
clarity and accuracy.
7-8 points

Outline missing several of the
required components, and/orplanned experiment does not meet
several of the requirements for the assignment and/ormajor
issues with clarity and accuracy.
5-6 points

Outline missing most of the
required components andplanned experiment does not meet the
requirements of the assignment.
0-4 points

Introduction, Question and
Hypothesis (15 points)

Background information about
enzymes in general and about specific enzyme used in project is clearly and
accurately written. Questions and hypothesis are specific, relevant and
clearly stated.
14-15 points

Background information about
enzymes in general and about specific enzyme is somewhat unclear and/or
inaccurate. Questions and hypothesis could be more specific, relevant and
clearly stated.
11-13 points

Missing background information
about enzymes in general orabout specific enzyme used in
project orquestions and hypothesis.
7-12 point

Missing background information
about enzymes in general andabout specific enzyme used in
project andquestions/hypothesis.
0-6 points

Experimental Design, Materials
and Methods (20 points)

Experiment is designed to
directly test the hypothesis; description of experiment is detailed and
well written and includes all materials and methods used.

18-20 points

Experiment is mostly designed to
test the hypothesis and/ordescription of experiment is somewhat
inaccurateand/or some information about materials and methods
used is missing and/orminor problems with clarity an
organization.
15-17 points

Experiment is barely designed to
test the hypothesis and/ordescription of experiment is
inaccurate and/or most of the materials and methods used
are missing and/or major problems with clarity an
organization.
10-14 points

Experiment is not designed to
test hypothesis and/ordescription of experiment and materials
and methods used are missing.
0-9 points

Results (20 points)

Results are clearly and
accurately presented in a table and/or graph format.
18-20 points

Results are presented, but minor
problems with clarity and/or accuracy.
15-17 points

Results are described, but major
problems with clarity and/or accuracy and/or results not presented
in table or graph.
10-14 points

Results are not included

0-9 points

Discussion of Results (15
points)

A clear, accurate and well
organized discussion of results that demonstrates good knowledge of
enzymes.
14-15 points

Minor problems with clarity,
accuracy or organization and/ordemonstrates some gaps in
knowledge of enzymes.
15-17 points

Major problems with clarity,
accuracy or organization ordemonstrates minimal knowledge of
enzymes.
10-14 points

Missing discussion of results,
or major problems with clarity, accuracy or organization andmissing
understanding of enzymes.
0-9 points

Conclusion ( 10 points )

A well stated conclusion that is
accurate and specific and directly related to the data collected. Clearly
stated whether or not the results support the hypothesis. Relevant
suggestion for improvement of experiment.
9-10 points

Conclusion stated, but slightly
inaccurate,and/or not directly related to data collected and/orsuggestion
for improvement of experiment not very relevant.
7-8 points

Conclusion stated, but
inaccurate and/orbarely related to data collected and/ormissing
suggestion for improvement of experiment.
5-6 points

Missing conclusion, only
suggestion for improvement of experiment.

0-4 points

Citations (5 points)

All references used are included
and in correct APA format, in-text references are included.
5 points

All references are included, but
a few minor mistakes in APA format, and/or missing in-text
references.
4 points

Missing one or several
references and/or major mistakes in APA format.
3 point

No references included or
references not in APA format.

0-2 points

Grammar and Spelling (5 points)

Less than 3 minorspelling
and grammatical errors
5 points

Between 4 and 7 minor spelling
and grammatical errors
4 points

Between 8 and 12 spelling and
grammatical errors
3 point

More than 13 spelling and
grammatical errors
0-2 points

Overall Score

Level 4
90 or more

Level 3
75 or more

Level 2
50 or more

Level 1
0 or more