Points: \t\/10<\/p>\n
Name:\tSection:\tGrade: \/10<\/p>\n
Format – Best Help Writing My 99 Papers\u2014owl Essay Samplesting:
\nKEEP THE ORIGINAL FORMATTING only use the tables available to report your results, insert text, graphs, and calculations where you are directed.
\nCheck with your lab instructor about how you need to submit your worksheet. You will be asked to either hand in a paper copy or submit it electronically. Electronic submissions are best in a PDF to preserve formatting!
\nFailing the above WILL RESULT IN LOST OF MARKS (up to 1 pt.))<\/p>\n
1.\tTLC Data analysis
\nTLC data\t2 pts.<\/p>\n
Include a figure representing a picture of your TLC plate. Label your figure to clearly show the Mono- Di- and Trioline bands in the Standard and Fish oil lanes. Follow the figure-making guide for the accepted formatting.
\nInsert figure here<\/p>\n
Amount of neutral lipid in the fish oil tested\t3 pts.<\/p>\n
1.\tPick one type of neutral lipid to quantify in the fish oil (i.e. Mono- or Di- or Trioline), indicate the identity of the lipid in table
\n2.\tCalculate the \u00b5g of neutral lipid loaded in each standard lane.
\n3.\tUsing Image J, measure the intensity of the chosen lipid\u2019s band in the standard mixture and fish oil lanes.<\/p>\n
Neutral lipid identity (i.e. Mono- Di- or Trioline):
\nSample \t\u00b5g Lipid loaded\tIntensity
\nStandard 1
\nStandard 2
\nStandard 3
\nFish Oil 1
\nFish Oil 2<\/p>\n
4.\tMake the lipid Standard curve by plotting intensity\/area of peak versus the \u00b5g of lipid loaded per lane in the three standard lanes, (report the standard plot in the supplementary info (do not need to have a caption just a well-labelled figure).
\n5.\tUse the slope of the curve to calculate the amount of neutral lipid in the original fish oil sample.
\nShow calculations here<\/p>\n
2. MS Data Analysis
\nAnswer specific questions regarding an MS dataset collected from fish oil samples. The MS Data is presented in the following figures. \t5 pts.<\/p>\n
Figure 1, Figure 2, Figure 3
\nFor your calculations, assume the mass of C, O and H atoms are 12, 16 and 1, respectively. Show all your calculations!
\n1)\tWhat would the m\/Z be for the neutral lipid you chose to quantify in the TLC experiment with 1 and 3 ammonium ions bound to it? Can you find a peak on any of the figures? (1 pt)
\nAdd answer here
\n2)\tFigure 2 shows an enlargement of the region which can be interpreted to contain long-chain triacylglycerols (~800-1000 m\/Z). – – One distinct feature is that the more intense peaks (i.e., higher relative abundance) have m\/Z values separated by \u00b12 m\/Z from neighbouring intense peaks. Suggest what this may be a result of. Also, explain the source of the \u201cgrouping\u201d of these peaks into clusters separated by \u00b1~28 from the neighbouring clusters (particularly visible in canola and halibut oil spectra). (Hint: Z = +1 for these species). (1 pt)
\nAdd answer here
\n3)\tFigure 3 shows an enlargement of the region containing the medium-chain triacylglycerols. Consider the peak at m\/Z 516 again.
\na)\tAssuming that that peak has a +1 charge and that the charge comes from an ammonium ion, what is this lipid’s \u201creal\u201d mass? (1 pt)
\nb)\tAssuming it is a triacylglycerol with fully saturated fatty acids, draw a suggested structure of this molecule Show all your calculations! (1 pt)
\nc)\tIs your suggested structure the ONLY possible structure consistent with the mass, and why? (1 pt)
\nAdd answer here
\nSupplementary information<\/p>\n
Insert the standard curve here
\n++++
\nBCEM2201<\/p>\n
TLC Data analysis
\nFigure 1: TLC plate with Mono-, Di-, and Trioline bands in the Standard and Fish oil lanes.
\nTLC plate<\/p>\n
Neutral lipid identity: Dioline<\/p>\n
Sample\t\u00b5g Lipid loaded\tIntensity
\nStandard 1\t40\t1569
\nStandard 2\t60\t2291
\nStandard 3\t80\t3135
\nFish Oil 1\tN\/A\t1642
\nFish Oil 2\tN\/A\t1593
\nCalculations:<\/p>\n
\u00b5g lipid loaded per lane = (amount of lipid (mg)\/volume of solution (\u00b5L)) x volume spotted (\u00b5L) x 1000
\n\u00b5g Dioline loaded per lane in Standards 1-3 = (10\/100) x 1 x 1000 = 40, (15\/100) x 1 x 1000 = 60, (20\/100) x 1 x 1000 = 80
\nAmount of Dioline in Fish Oil = (Intensity of Fish Oil \/ Slope of Standard Curve) x \u00b5g Dioline loaded per lane in Standards 2 = (1642 \/ 0.0456) x 60 = 2196 \u00b5g
\nMS Data Analysis
\nThe m\/Z for Dioline with 1 and 3 ammonium ions bound to it would be:
\nDioline with 1 ammonium ion: [(2 x 12) + (36) + (1 x 22) + (1 x 15)] \/ 1 = 87
\nDioline with 3 ammonium ions: [(2 x 12) + (36) + (1 x 22) + (3 x 15)] \/ 3 = 103
\nThere is a peak at m\/Z 87 in Figure 1 (Fish Oil) that could correspond to Dioline with 1 ammonium ion bound to it.
\nThe grouping of peaks separated by \u00b12 m\/Z from neighbouring intense peaks in Figure 2 may be a result of the presence of different isotopes of the same molecule, each with a slightly different mass. The peaks are grouped into clusters separated by \u00b1~28 from the neighbouring clusters due to the presence of different types of fatty acids, which have different masses and contribute differently to the overall mass of the molecule.<\/p>\n
a) The \u201creal\u201d mass of the peak at m\/Z 516 in Figure 3, assuming a +1 charge and an ammonium ion, would be [(3 x 12) + (3 x 16) + (3 x 1) – (1 x 15)] \/ 1 = 165. Therefore, the lipid at m\/Z 516 would have a \u201creal\u201d mass of 165 Da.
\nb) A suggested structure for this molecule would be triolein, which is a triacylglycerol composed of three oleic acid molecules. Oleic acid has a chain length of 18 carbons, which contributes a mass of [(18 x 12) + (32 x 1) + (1 x 16)] = 282 to the molecule. Therefore, the suggested structure for the lipid at m\/Z 516 would be:<\/p>\n
css
\nCopy code
\nO
\n|||
\nO-C-C-C-O-C-C-C-O-C-C-C-O
\n| | | | | | | | | | |
\nH-H-H-H-H-H-H-H-H-H-H-H
\nc) No, the suggested structure<\/p>\n","protected":false},"excerpt":{"rendered":"
Points: \/10 Name: Section: Grade: \/10 Format – Best Help Writing My 99 Papers\u2014owl Essay Samplesting: KEEP THE ORIGINAL FORMATTING only use the tables available\u2026<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[361],"tags":[3262,2619],"class_list":["post-6743","post","type-post","status-publish","format-standard","hentry","category-medical-science-assignment-help","tag-bcem2201-tlc-data-analysis","tag-help-write-my-paper"],"_links":{"self":[{"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/posts\/6743","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/comments?post=6743"}],"version-history":[{"count":1,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/posts\/6743\/revisions"}],"predecessor-version":[{"id":6744,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/posts\/6743\/revisions\/6744"}],"wp:attachment":[{"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/media?parent=6743"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/categories?post=6743"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.colapapers.com\/uk\/wp-json\/wp\/v2\/tags?post=6743"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}