Question description<\/p>\n
Assignment:\u00a0In this lab, we are comparing sequences of phosphoglycerate kinase amongst different organisms.\u00a0Procedure:1. Visit\u00a0http:\/\/www.ebi.ac.uk\/Tools\/msa\/clustalo\/2. Copy & Paste alignment sequences into input box (You will find this attached as “4NG4 Multisequence”)3. Click Submit4. Right Click “Download Alignment File” –> “Download Linked File”5. Visit\u00a0http:\/\/consurf.tau.ac.il6. Click Amino Acids –> Is there a protein structure ? Yes –> PDB ID: 4NG4 –> Click “Next” –> Chain A –> MSA to upload ? Yes –> Choose File: Upload the file you downloaded from Clustal Omega –> Query Sequence Name:\u00a0sp|P0A799|PGK_ECOLI –> Tree file to upload ? No –> Uncheck send link to email –> Click “Submit” & wait for resultsAfter getting results from Consurf, use Jmol or Chimera to:\u00a01) produce the overall 3D structure (capture\/screenshot this image)2) produce an image of least conserved regions and most conserved regions\u00a0(capture\/screenshot this image)***NOTE:\u00a0The molecule will be in space filling mode color coded by conservation with the most conserved regions in maroon and the least conserved regions in blue. ***ALSO, If Jmol doesn’t work you may have to download Chimera !<\/p>\n","protected":false},"excerpt":{"rendered":"
Question description Assignment:\u00a0In this lab, we are comparing sequences of phosphoglycerate kinase amongst different organisms.\u00a0Procedure:1. Visit\u00a0http:\/\/www.ebi.ac.uk\/Tools\/msa\/clustalo\/2. Copy & Paste alignment sequences into input box (You will find this attached as “4NG4 Multisequence”)3. Click Submit4. Right Click “Download Alignment File” –> “Download Linked File”5. Visit\u00a0http:\/\/consurf.tau.ac.il6. Click Amino Acids –> Is there a protein structure ? Yes […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,6515,8569,8567,8568,8570],"tags":[5882,6803,4009,7294,7293],"class_list":["post-48017","post","type-post","status-publish","format-standard","hentry","category-dissertation-writing","category-essay-writing","category-homework-assistance","category-online-assignment-help","category-paper-writing-services","category-write-my-essay-2","tag-best-essays-uk","tag-dissertation-topic-help","tag-do-my-essay-assignment-in-ireland","tag-essay-for-sale-australia","tag-help-with-writing-an-essay"],"_links":{"self":[{"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/posts\/48017","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/comments?post=48017"}],"version-history":[{"count":0,"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/posts\/48017\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/media?parent=48017"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/categories?post=48017"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.colapapers.com\/us\/wp-json\/wp\/v2\/tags?post=48017"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}